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rnascope fluorescence in situ hybridization (fish)  (Jackson Laboratory)

 
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    Jackson Laboratory rnascope fluorescence in situ hybridization (fish)
    Rnascope Fluorescence In Situ Hybridization (Fish), supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rnascope fluorescence in situ hybridization (fish)/product/Jackson Laboratory
    Average 90 stars, based on 1 article reviews
    rnascope fluorescence in situ hybridization (fish) - by Bioz Stars, 2026-03
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    Localization of angiotensinogen (Agt) within the arcuate nucleus of the hypothalamus (ARC) and confirmation of cell-specific deletion of Agt in AgtLepr-KO and AgtAgrp-KO mice [mice exhibiting selective deletion of the Agt gene in cells that express either leptin receptor (Lepr) or agouti-related peptide (Agrp), respectively]. A: colocalization of Agt mRNA with mRNA for glial fibrillary acidic protein (Gfap), Lepr, proopiomelanocortin (Pomc), insulin II (Ins2), or Agrp in the ARC from a wild-type C57BL/6J mouse using in situ <t>hybridization/RNAscope.</t> DAPI, 4′,6-diamidino-2-phenylindole nuclear stain; ME, median eminence; 3V, third ventricle. B: loss of colocalization of Agt mRNA with Lepr in AgtLepr-KO mice. Black arrows identify a Lepr-positive (panel at left) but Agt-negative cell, whereas white arrows identify an Agt-positive, Gfap-positive neighboring cell. C: loss of colocalization of Agt mRNA with Agrp in AgtAgrp-KO mice. The black arrow identifies an Agrp-/Lepr-positive + Agt-negative cell, whereas the white arrow identifies an Agt-positive + Agrp-/Lepr-negative neighboring cell.
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    Localization of angiotensinogen (Agt) within the arcuate nucleus of the hypothalamus (ARC) and confirmation of cell-specific deletion of Agt in AgtLepr-KO and AgtAgrp-KO mice [mice exhibiting selective deletion of the Agt gene in cells that express either leptin receptor (Lepr) or agouti-related peptide (Agrp), respectively]. A: colocalization of Agt mRNA with mRNA for glial fibrillary acidic protein (Gfap), Lepr, proopiomelanocortin (Pomc), insulin II (Ins2), or Agrp in the ARC from a wild-type C57BL/6J mouse using in situ <t>hybridization/RNAscope.</t> DAPI, 4′,6-diamidino-2-phenylindole nuclear stain; ME, median eminence; 3V, third ventricle. B: loss of colocalization of Agt mRNA with Lepr in AgtLepr-KO mice. Black arrows identify a Lepr-positive (panel at left) but Agt-negative cell, whereas white arrows identify an Agt-positive, Gfap-positive neighboring cell. C: loss of colocalization of Agt mRNA with Agrp in AgtAgrp-KO mice. The black arrow identifies an Agrp-/Lepr-positive + Agt-negative cell, whereas the white arrow identifies an Agt-positive + Agrp-/Lepr-negative neighboring cell.
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    rnascope fluorescence in situ hybridization (fish)  (Jackson Laboratory)
    90
    Jackson Laboratory rnascope fluorescence in situ hybridization (fish)
    Localization of angiotensinogen (Agt) within the arcuate nucleus of the hypothalamus (ARC) and confirmation of cell-specific deletion of Agt in AgtLepr-KO and AgtAgrp-KO mice [mice exhibiting selective deletion of the Agt gene in cells that express either leptin receptor (Lepr) or agouti-related peptide (Agrp), respectively]. A: colocalization of Agt mRNA with mRNA for glial fibrillary acidic protein (Gfap), Lepr, proopiomelanocortin (Pomc), insulin II (Ins2), or Agrp in the ARC from a wild-type C57BL/6J mouse using in situ <t>hybridization/RNAscope.</t> DAPI, 4′,6-diamidino-2-phenylindole nuclear stain; ME, median eminence; 3V, third ventricle. B: loss of colocalization of Agt mRNA with Lepr in AgtLepr-KO mice. Black arrows identify a Lepr-positive (panel at left) but Agt-negative cell, whereas white arrows identify an Agt-positive, Gfap-positive neighboring cell. C: loss of colocalization of Agt mRNA with Agrp in AgtAgrp-KO mice. The black arrow identifies an Agrp-/Lepr-positive + Agt-negative cell, whereas the white arrow identifies an Agt-positive + Agrp-/Lepr-negative neighboring cell.
    Rnascope Fluorescence In Situ Hybridization (Fish), supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    rnascope fluorescence in situ hybridization (fish) - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Localization of angiotensinogen (Agt) within the arcuate nucleus of the hypothalamus (ARC) and confirmation of cell-specific deletion of Agt in AgtLepr-KO and AgtAgrp-KO mice [mice exhibiting selective deletion of the Agt gene in cells that express either leptin receptor (Lepr) or agouti-related peptide (Agrp), respectively]. A: colocalization of Agt mRNA with mRNA for glial fibrillary acidic protein (Gfap), Lepr, proopiomelanocortin (Pomc), insulin II (Ins2), or Agrp in the ARC from a wild-type C57BL/6J mouse using in situ hybridization/RNAscope. DAPI, 4′,6-diamidino-2-phenylindole nuclear stain; ME, median eminence; 3V, third ventricle. B: loss of colocalization of Agt mRNA with Lepr in AgtLepr-KO mice. Black arrows identify a Lepr-positive (panel at left) but Agt-negative cell, whereas white arrows identify an Agt-positive, Gfap-positive neighboring cell. C: loss of colocalization of Agt mRNA with Agrp in AgtAgrp-KO mice. The black arrow identifies an Agrp-/Lepr-positive + Agt-negative cell, whereas the white arrow identifies an Agt-positive + Agrp-/Lepr-negative neighboring cell.

    Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

    Article Title: Exploration of cardiometabolic and developmental significance of angiotensinogen expression by cells expressing the leptin receptor or agouti-related peptide

    doi: 10.1152/ajpregu.00297.2019

    Figure Lengend Snippet: Localization of angiotensinogen (Agt) within the arcuate nucleus of the hypothalamus (ARC) and confirmation of cell-specific deletion of Agt in AgtLepr-KO and AgtAgrp-KO mice [mice exhibiting selective deletion of the Agt gene in cells that express either leptin receptor (Lepr) or agouti-related peptide (Agrp), respectively]. A: colocalization of Agt mRNA with mRNA for glial fibrillary acidic protein (Gfap), Lepr, proopiomelanocortin (Pomc), insulin II (Ins2), or Agrp in the ARC from a wild-type C57BL/6J mouse using in situ hybridization/RNAscope. DAPI, 4′,6-diamidino-2-phenylindole nuclear stain; ME, median eminence; 3V, third ventricle. B: loss of colocalization of Agt mRNA with Lepr in AgtLepr-KO mice. Black arrows identify a Lepr-positive (panel at left) but Agt-negative cell, whereas white arrows identify an Agt-positive, Gfap-positive neighboring cell. C: loss of colocalization of Agt mRNA with Agrp in AgtAgrp-KO mice. The black arrow identifies an Agrp-/Lepr-positive + Agt-negative cell, whereas the white arrow identifies an Agt-positive + Agrp-/Lepr-negative neighboring cell.

    Article Snippet: Coronal sections of 10 μm were cut using a cryostat, and sections underwent RNAscope Multiplex fluorescence in situ hybridization (FISH) for fresh frozen samples (Advanced Cell Diagnostics).

    Techniques: In Situ Hybridization, RNAscope, Staining

    Fluorescent in situ hybridization (RNAscope) detection of endogenous angiotensinogen (Agt) and agouti-related peptide (Agrp) mRNA in adrenal gland of a wild-type mouse. A: in situ hybridization to localize nuclei (DAPI, blue), Agt (green), and Agrp (red) in adrenal gland of a wild-type female mouse. B: image from inset of A, shown at greater magnification. C: similar in situ hybridization in adrenal gland of a wild-type male mouse. D: image from inset of C, shown at greater magnification.

    Journal: American Journal of Physiology - Regulatory, Integrative and Comparative Physiology

    Article Title: Exploration of cardiometabolic and developmental significance of angiotensinogen expression by cells expressing the leptin receptor or agouti-related peptide

    doi: 10.1152/ajpregu.00297.2019

    Figure Lengend Snippet: Fluorescent in situ hybridization (RNAscope) detection of endogenous angiotensinogen (Agt) and agouti-related peptide (Agrp) mRNA in adrenal gland of a wild-type mouse. A: in situ hybridization to localize nuclei (DAPI, blue), Agt (green), and Agrp (red) in adrenal gland of a wild-type female mouse. B: image from inset of A, shown at greater magnification. C: similar in situ hybridization in adrenal gland of a wild-type male mouse. D: image from inset of C, shown at greater magnification.

    Article Snippet: Coronal sections of 10 μm were cut using a cryostat, and sections underwent RNAscope Multiplex fluorescence in situ hybridization (FISH) for fresh frozen samples (Advanced Cell Diagnostics).

    Techniques: In Situ Hybridization, RNAscope